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Removing DNA-fragments from indoor air in PCR laboratories

Develops innovative molecular solutions to advance the diagnosis of infectious diseases and antibiotic resistances

Established in 2000 in Finland, Mobidiag has offices in France, UK and Sweden.

Challenge

Mobidiag Inc. is a company developing and manufacturing molecular diagnostics platforms and test kits for detecting human pathogens. The products utilize PCR technology, where target DNA segments are amplified, generating thousands to millions of copies of a particular DNA sequence. Due to this, the air decontamination in the laboratory environment is extremely important.

”The challenge in air decontamination is not only the traditional particle purification, but for us the most critical part is the purification of the amplification product that we are making ourselves in the laboratory”, describes CEO Tuomas Tenkanen (photo).

“A contamination of our samples or products with amplification products could cause wrong positive results in our diagnostics tests. The PCR amplification products (amplicons) are very difficult targets for air purification, since their size is small. The size of these is from 100 to 500 base pairs which equals 30–200 nanometers. Traditional HEPA filters are not capable to filter those amplicons or the capability is limited”, he adds.

“The PCR amplification products are very difficult targets for air purification, since their size is small. Genano system is a perfect solution for our needs.” – CEO Tuomas Tenkanen.

Solution

The air brought to the laboratories is decontaminated by a Genano Air Decontamination Unit before it is lead in the room. In addition, stand-alone Genano units are used to circulate and decontaminate the air within critical zones. Ventilation was adjusted to achieve the right pressure differencial between laboratory areas in order to prevent airborne particles and contaminants from moving towards cleaner areas.

“The PCR amplification products are very difficult targets for air purification, since their size is small. Genano system is a perfect solution for our needs”, summarizes Mr. Tenkanen.

Result

According to ISO 14464-1 airborne particle measurements were conducted after installing Genano Air Decontamination Units:

  • The laboratory has now two areas where the particle count is ISO 6 compliant.
  • The air in these laboratories is 96 % cleaner compared to the central area (sampling of 0.3 µm particles).

This level guarantees the good quality and reliability of solutions that Mobidiag offers. “Genano system is a perfect solution for our needs”, summarizes Mr. Tenkanen.